Protein Sequencing and Identification Using Tandem Mass Spectrometry

This volume describes the complete process of analyzing protein sequences by mass spectrometry including sample preparation, analysis, and data interpretation at a practical level. It provides sufficient experimental detail to carry out the experiments and consistently achieve good results.
Die rasche Entwicklung der Proteinsequenzierung innerhalb der letzten zwei bis drei Jahre ist vor allem drei Aspekten zu verdanken: der Anwendung neuartiger Verfahren zum Proteinabbau, der Erweiterung der DNA-Sequenz-Datenbanken (z. B. vom National Center for Biotechnology Information) und der Verfugbarkeit von Computerprogrammen zum effizienten Durchsuchen dieser Datenbestande. Dieser hochaktuelle erlautert den gesamten Proze? der Proteinsequenzierung durch Massenspektrometrie - von der Probenvorbereitung und Analyse bis zur Interpretation. Mit vielen praxisnahen Tipps! (11/00)

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