In-situ PCR is an attractive alternative to standard and fluorescence in-situ hybridization (F.I.S.H. ) that provides greatly enhanced sensitivity. This book presents protocols - tested and validated for sensitivity, precision and reproducibility - for in-situ PCR detection and localization of RNA and DNA.
This book describes comprehensive step-by-step protocols for the delineation of genetic amplification and histological detection techniques. Each procedure has been tested and validated for its sensitivity, precision, and reproducibility, and the authors give advice on the design of primers for PCR applications and on optimizing these protocols for use with plant, insect, and prokaryotic cells.